ncode™ mirna first-strand cdna synthesis qrt-pcr kit (Thermo Fisher)
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Thermo Fisher
ncode™ mirna first-strand cdna synthesis qrt-pcr kit
Ncode™ Mirna First Strand Cdna Synthesis Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+qrt-pcr+kits/Single+Cell-to-CT+qRT-PCR+Kit/pmc07053300-86-27-29
Average 98 stars, based on 888 article reviews
Ncode™ Mirna First Strand Cdna Synthesis Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+qrt-pcr+kits/Single+Cell-to-CT+qRT-PCR+Kit/pmc07053300-86-27-29
Average 98 stars, based on 888 article reviews
ncode™ mirna first-strand cdna synthesis qrt-pcr kit - by Bioz Stars,
2026-10
98/100 stars
Images
1) Product Images from "Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells"
Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
Journal: International Journal of Medical Sciences
doi: 10.7150/ijms.38832
Figure Legend Snippet: MiR-20b-5p and miR-106a-5p and oxidative stress regulate specific genes of the p21/CDK/E2F pathway. (A) 3'-UTR of the transcripts of genes of the p21 pathway; the predicted miR-20b-5p/miR-106a-5p target sites (vertical green bar) are shown. Bent arrows indicate the position of the PCR primers used in the amplification of the 3'UTR segments (boxed) cloned into the pmirGLO vector . (B) Validation of targeting of miR-20b-5p/miR-106a-5p by luciferase assays. HCT-15 cells were co-transfected with pmirGLO/3'UTR or the pmirGLO blank vector, and a miR-20b-5p or miR-106a-5p mimic, or a negative control mimic. * p <0.05 and ** p <0.01 were relative to the data of pmirGLO/3'UTR without transfected miRNA or negative control (NC) mimic. (C) Inverse relationship between expression levels of miR-20b-5p/miR-106a-5p and the target transcripts and proteins in miRNA mimic-transfected cells, determined by qRT-PCR (left panel) and western blot analysis (right panel). (D) Effects of oxidative stress on the miRNA target transcript and protein levels in three MSC cell lines. The total RNA (left panel) and protein samples (right panel) were prepared from H 2 O 2 -treated (2 h) or -untreated cells, which were further cultured for 24 h post H 2 O 2 treatment before being harvested for further analysis. * p <0.05 and ** p <0.01 were relative to the untreated or the negative control (NC) samples. In the western blots in (C) and (D) : RL, relative levels compared to the negative control (C) or untreated control (D) samples.
Techniques Used: Amplification, Clone Assay, Plasmid Preparation, Luciferase, Transfection, Negative Control, Expressing, Quantitative RT-PCR, Western Blot, Cell Culture
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Polyadenylation of miRNAs from total RNA and synthesis of Expressing:Article Title: Definition of miRNAs Expression Profile in Glioblastoma Samples: The Relevance of Non-Neoplastic Brain Reference Article Snippet: .. Briefly, RNA was retro-transcribed using the Real-time Polymerase Chain Reaction:Article Title: Regulation of atrophin by both strands of the mir-8 precursor. Article Snippet: In Drosophila melanogaster, miR-8-3p regulates mRNA levels of atrophin, a factor involved in neuromotor coordination, and we found that Blattella germanica with suppressed atrophin showed motor problems.. Bionformatic predictions and luciferase-reporter tests indicated that B. germanica atrophin mRNA contains target sites for miR-8-3p and miR-8-5p.. Suppression of miR-8-3p or miR-8-5p appeared to increase atrophin mRNA. Article Title: Serum Response Factor Regulates Expression of Phosphatase and Tensin Homolog Through a MicroRNA Network in Vascular Smooth Muscle Cells Article Snippet: Sequence-specific primers were designed using the MacVector program (Macvector, NC): PTEN: sense (5’-ccttttgaagaccataacccacc-3’), antisense (5’-ttacaccagtccgtcctttcccag-3’); SM-α-actin: sense (5’-ccagccagtcgccatcag-3’), antisense (5’-agcatcatcaccagcaaagc-3’); MCP-1: sense (5’- tgctgtctcagccagatgcagtta-3’), antisense (5’-tacagcttctttgggacacctgct-3’); CXCL1/KC: sense (5’-ctaaaaggtgtccccaagtaac-3’), antisense (5’-attgtatagtgttgtcagaagcc-3’); β-actin: sense (5’-agggtgtgatggtgggtatgg-3’), antisense (5’-aatgccgtgttcaatgggg-3’). .. 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Total RNA was polyadenylated and reverse transcribed for use in a two-step qRT-PCR using the Quantitation Assay:Article Title: Serum Response Factor Regulates Expression of Phosphatase and Tensin Homolog Through a MicroRNA Network in Vascular Smooth Muscle Cells Article Snippet: Sequence-specific primers were designed using the MacVector program (Macvector, NC): PTEN: sense (5’-ccttttgaagaccataacccacc-3’), antisense (5’-ttacaccagtccgtcctttcccag-3’); SM-α-actin: sense (5’-ccagccagtcgccatcag-3’), antisense (5’-agcatcatcaccagcaaagc-3’); MCP-1: sense (5’- tgctgtctcagccagatgcagtta-3’), antisense (5’-tacagcttctttgggacacctgct-3’); CXCL1/KC: sense (5’-ctaaaaggtgtccccaagtaac-3’), antisense (5’-attgtatagtgttgtcagaagcc-3’); β-actin: sense (5’-agggtgtgatggtgggtatgg-3’), antisense (5’-aatgccgtgttcaatgggg-3’). .. 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Quantitative real-time PCR was performed as previously described and β-actin was used for normalization. miRNA Quantitation. miRNA was isolated from VSMC using the mirVana miRNA isolation kit (Ambion, TX) and first strand cDNA was made using the Article Title: Effects of miR-9 and tetramethylpyrazine on activation of hepatic stellate cells. Article Snippet: .. All the cells were collected and stored at −80°C until use. miRNA Isolation and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) for miRNA Assay miRNA was isolated from LX-2 cell using the mirVana miRNA isolation kit (Ambion, TX, U.S.A.) and first-strand cDNA was synthesized using the Reverse Transcription Polymerase Chain Reaction:Article Title: Effects of miR-9 and tetramethylpyrazine on activation of hepatic stellate cells. Article Snippet: .. All the cells were collected and stored at −80°C until use. miRNA Isolation and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) for miRNA Assay miRNA was isolated from LX-2 cell using the mirVana miRNA isolation kit (Ambion, TX, U.S.A.) and first-strand cDNA was synthesized using the Synthesized:Article Title: Effects of miR-9 and tetramethylpyrazine on activation of hepatic stellate cells. Article Snippet: .. All the cells were collected and stored at −80°C until use. miRNA Isolation and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) for miRNA Assay miRNA was isolated from LX-2 cell using the mirVana miRNA isolation kit (Ambion, TX, U.S.A.) and first-strand cDNA was synthesized using the SYBR Green Assay:Article Title: Akt Protein Kinase, miR-200/miR-182 Expression and Epithelial-Mesenchymal Transition Proteins in Hibernating Ground Squirrels Article Snippet: Total RNA, including small RNAs, was purified from powdered TLGS brains, kidneys, hearts, or cultured SHSY5Y cells using the mirVana miRNA isolation kit (Invitrogen). .. Polyadenylation of miRNAs from total RNA and synthesis of other:Article Title: Cross-kingdom microRNA transfer for the control of the anthracnose disease in cassava Article Snippet: Cassava anthracnose disease (CAD), caused by Colletotrichum gloeosporioides f. sp. manihotis, is one of the most important diseases that cause significant yield loss in cassava.. Recently, involvement of microRNAs (miRNAs), a class of small, single-stranded, non-coding RNAs, in the resistance against anthracnose disease has been indicated.. In this study, two cassava cultivars that have different degree of CAD susceptibility were utilized to investigate the differences in defense responses these cultivars exhibited and to examine the role of seven miRNA families (mes-MIR156, 159, 164, 171, 396, 408, and 530) during CAD infection. |
